Review

human keratinocyte growth supplement (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher human keratinocyte growth supplement
Human Keratinocyte Growth Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+keratinocyte+growth+supplement/Transferrin/pmc12346516-188-14-19
Average 99 stars, based on 1 article reviews

human keratinocyte growth supplement - by Bioz Stars, 2026-10

99/100 stars

Images

Cell Culture:

Article Title: RNA Sequencing Reveals the Long Non-Coding RNA Signature in Psoriasis Keratinocytes and Identifies CYDAER as a Long Non-Coding RNA Regulating Epidermal Differentiation.
Article Snippet: .. Primary human epidermal keratinocytes (Thermo Fisher Scientific, Waltham, MA, USA) were cultured in Epilife Growth medium supplemented with 1% Penicillin/Streptomycin antibiotics and 1% Human Keratinocyte Growth Supplement (both Gibco, Waltham, MA, USA). .. Primary keratinocytes were treated with human recombinant IL- 17A (100 ng/mL) (R&D Systems, Minneapolis, MN, USA).

Article Title: Anti-melanogenic effects of hydroxyethyl chrysin through the inhibition of tyrosinase activity: In vitro and in silico approaches
Article Snippet: .. Normal human primary epidermal keratinocytes (NHEK; PCS-200-011, ATCC) were cultured in 154CF medium (Gibco BRL) supplemented with 1 × Human Keratinocyte Growth Supplement (Gibco BRL), 0.07 mM CaCl 2 (Gibco BRL), and 100 U/mL penicillin/streptomycin, at 37 °C with 5 % CO 2 . .. A WST-1 assay was performed to assess cell cytotoxicity [ ] using an EZ-Cytox cell viability assay kit (DAEIL Lab, Seoul, Korea).

Article Title: Per- and Polyfluoroalkyl Substance-Induced Skin Barrier Disruption and the Potential Role of Calcitriol in Atopic Dermatitis
Article Snippet: Human Epidermal Keratinocyte, neonatal (HEKn) cells were purchased from GibcoTM (Thermo Fisher Scientific, Waltham, MA, USA). .. HEKn cells were cultured according to the manufacturer’s instructions in EpiLifeTM Medium supplemented with Human Keratinocyte Growth Supplement (HKGS, Gibco; containing 0.2% v/v bovine pituitary extract, 0.01 μg/mL recombinant human insulin-like growth factor-I, 0.18 μg/mL hydrocortisone, 5 μg/mL bovine transferrin, and 0.2 ng/mL human epidermal growth factor; Gibco) and 60 μM calcium. ..

Isolation:

Article Title: Transcriptional and functional regulation of cell cycle and UV response by PPARβ in human skin epidermal cells
Article Snippet: .. Normal human epidermal keratinocytes (NHEK, A13401 Invitrogen), isolated from multiple neonatal foreskins were maintained in EpiLifeTM Medium (MEPI500CA, Invitrogen) supplemented with human keratinocyte growth supplement (HKGS, S0015, Invitrogen), and penicillin–streptomycin 10 U/mL. ..

Suspension:

Article Title: Non-invasive in vitro NAM for the detection of reversible and irreversible eye damage after chemical exposure for GHS classification purposes (ImAi).
Article Snippet: .. Subsequently, the cell suspension was centrifuged at 300 g for 5 min, and the resulting pellet was resuspended in E2 medium (EpiLifeTM medium (Life Technologies, Carlsbad, USA) supplemented with 1%-human keratinocyte growth supplement (Life Technologies, Carlsbad, USA), 1%-penicillin/streptomycin (P/S) (Sigma-Aldrich, Schnelldorf, Germany), 0.48% 300-mM CaCl2 (Sigma-Aldrich, Schnelldorf, Germany)). ..

Recombinant:

other:

Article Title: Acetyl-CoA synthesis in the skin is a key determinant of systemic lipid homeostasis
Article Snippet: Human keratinocyte growth supplement , Thermo Fisher , Cat#S0015.

Article Title: Failed Metabolic Adaptation to Stress Underlies Pathogenesis in a Heterozygous Genetic Disorder
Article Snippet: For experiments, keratinocytes were removed from feeders and plated at 80-90% confluency in M154 (Thermo Fisher Scientific) growth media supplemented with 0.07 mM CaCl2, human keratinocyte growth supplement (HKGS; Thermo Fisher Scientific), gentamicin and amphotericin B 24-36 hours prior to the experiment.

Image Search Results

a , Study schema depicting the discovery and validation phase (created with BioRender.com ). We applied scRNA-seq, RNAscope, novel PNI organoids, and survival analysis to define the tumor cellular niche and therapeutically actionable intercellular pathways of PNI pos cancers. b , Uniform manifold approximation and projection (UMAP) of the cSCC cellular atlas, including PNI-positive and PNI-negative tumors compared to normal skin (n=92,371). c , UMAP view of epithelial cells, fibroblasts, mast cells, T cells, B/Plasma cells, and melanocytes. d , UMAP plot for keratinocytes in PNI pos cSCC (n=4830). e , UMAP of different keratinocyte subpopulations involved in PNI pos cSCC.

Journal: bioRxiv

Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

doi: 10.1101/2025.06.23.661147

Figure Lengend Snippet: a , Study schema depicting the discovery and validation phase (created with BioRender.com ). We applied scRNA-seq, RNAscope, novel PNI organoids, and survival analysis to define the tumor cellular niche and therapeutically actionable intercellular pathways of PNI pos cancers. b , Uniform manifold approximation and projection (UMAP) of the cSCC cellular atlas, including PNI-positive and PNI-negative tumors compared to normal skin (n=92,371). c , UMAP view of epithelial cells, fibroblasts, mast cells, T cells, B/Plasma cells, and melanocytes. d , UMAP plot for keratinocytes in PNI pos cSCC (n=4830). e , UMAP of different keratinocyte subpopulations involved in PNI pos cSCC.

Article Snippet: Cells were maintained at 37°C with 5% CO2 in medium 154 (Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific).

Techniques: Biomarker Discovery, RNAscope, Clinical Proteomics

a , UMAP view of myeloid, endothelial, and parotid cells in cSCC. b , DvP score depicting the loss of differentiation programming in keratinocytes in PNI pos cSCC. P-value was calculated by mixed effect model adjusting for patient ID with the Satterthwaite method. c , UMAP of basal keratinocytes in PNI pos cSCC. d , Gene set enrichment analysis (GSEA) for a stress-like signature in TCGA tumors with known PNI status. P-value was calculated by permutation test.

Journal: bioRxiv

Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

doi: 10.1101/2025.06.23.661147

Figure Lengend Snippet: a , UMAP view of myeloid, endothelial, and parotid cells in cSCC. b , DvP score depicting the loss of differentiation programming in keratinocytes in PNI pos cSCC. P-value was calculated by mixed effect model adjusting for patient ID with the Satterthwaite method. c , UMAP of basal keratinocytes in PNI pos cSCC. d , Gene set enrichment analysis (GSEA) for a stress-like signature in TCGA tumors with known PNI status. P-value was calculated by permutation test.

Article Snippet: Cells were maintained at 37°C with 5% CO2 in medium 154 (Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific).

Techniques:

a , Left, circle plot analysis displaying cellular crosstalk via ligand ( KITLG ) expressed by keratinocytes/fibroblasts and receptor ( KIT ) present on mast cells in PNI pos cSCC. Right, scRNA-seq data for KITLG and KIT expression in different cellular populations of PNI pos cSCC. b , Expression of KITLG in keratinocytes and fibroblasts in cSCC patients. Wilcoxon rank sum test calculated the P-value . c , Enrichment of KITLG and mast cell infiltration gene signature in TCGA tumors with known PNI status. P-value calculated by linear regression after adjusting for cancer types. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. d , RNAscope duplex in situ hybridization of KITLG (teal), KIT (red), and counterstained cell nuclei (blue) in normal skin, PNI neg , and PNI pos cSCC. Scale bar=100μm, images are representative of n=5/group. e , RNAscope duplex in situ hybridization of KITLG (teal), KIT (red), and counterstained cell nuclei (blue) in normal biliary epithelium, PNI neg , and PNI pos CHOL. Scale bar=100μm, images are representative of n=5/group. f , RNAscope duplex in situ hybridization of KITLG (teal), KIT (red), and counterstained cell nuclei (blue) in normal oral mucosa, PNI neg , and PNI pos HNSC. Scale bar=100μm, images are representative of n=5/group.

Journal: bioRxiv

Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

doi: 10.1101/2025.06.23.661147

Figure Lengend Snippet: a , Left, circle plot analysis displaying cellular crosstalk via ligand ( KITLG ) expressed by keratinocytes/fibroblasts and receptor ( KIT ) present on mast cells in PNI pos cSCC. Right, scRNA-seq data for KITLG and KIT expression in different cellular populations of PNI pos cSCC. b , Expression of KITLG in keratinocytes and fibroblasts in cSCC patients. Wilcoxon rank sum test calculated the P-value . c , Enrichment of KITLG and mast cell infiltration gene signature in TCGA tumors with known PNI status. P-value calculated by linear regression after adjusting for cancer types. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. d , RNAscope duplex in situ hybridization of KITLG (teal), KIT (red), and counterstained cell nuclei (blue) in normal skin, PNI neg , and PNI pos cSCC. Scale bar=100μm, images are representative of n=5/group. e , RNAscope duplex in situ hybridization of KITLG (teal), KIT (red), and counterstained cell nuclei (blue) in normal biliary epithelium, PNI neg , and PNI pos CHOL. Scale bar=100μm, images are representative of n=5/group. f , RNAscope duplex in situ hybridization of KITLG (teal), KIT (red), and counterstained cell nuclei (blue) in normal oral mucosa, PNI neg , and PNI pos HNSC. Scale bar=100μm, images are representative of n=5/group.

Article Snippet: Cells were maintained at 37°C with 5% CO2 in medium 154 (Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific).

Techniques: Expressing, RNAscope, In Situ Hybridization

a , Copy number analysis of KITLG in primary human cSCC. Fisher’s exact test calculated P-value . b , Single-cell regulatory network inference and clustering (SCENIC)-based prediction of upstream regulators of KITLG in keratinocytes. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. c , Pathway activity scores of predicted KITLG regulators in PNI neg cSCC and KITLG -amplified and KITLG -diploid PNI pos tumors. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. d , IGV view of KITLG gene body with open chromatin, transcription regulators, and transcription factor binding analyzed using publicly available ChIP-seq data. e , Expression analysis of CXCL10 and KITLG upon STAT1 phosphorylation inhibition with fludarabine in keratinocytes. Conducted with replicates and repeated four independent times. Two-tailed Student’s t-tests calculated the P-value , error bar-standard deviation.

Journal: bioRxiv

Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

doi: 10.1101/2025.06.23.661147

Figure Lengend Snippet: a , Copy number analysis of KITLG in primary human cSCC. Fisher’s exact test calculated P-value . b , Single-cell regulatory network inference and clustering (SCENIC)-based prediction of upstream regulators of KITLG in keratinocytes. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. c , Pathway activity scores of predicted KITLG regulators in PNI neg cSCC and KITLG -amplified and KITLG -diploid PNI pos tumors. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. d , IGV view of KITLG gene body with open chromatin, transcription regulators, and transcription factor binding analyzed using publicly available ChIP-seq data. e , Expression analysis of CXCL10 and KITLG upon STAT1 phosphorylation inhibition with fludarabine in keratinocytes. Conducted with replicates and repeated four independent times. Two-tailed Student’s t-tests calculated the P-value , error bar-standard deviation.

Article Snippet: Cells were maintained at 37°C with 5% CO2 in medium 154 (Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific).

Techniques: Activity Assay, Amplification, Binding Assay, ChIP-sequencing, Expressing, Phospho-proteomics, Inhibition, Two Tailed Test, Standard Deviation

a , Expression of various mast cell mediator receptors in keratinocyte populations of PNI neg and PNI pos cSCC assessed by scRNA-seq. Wilcoxon rank sum test calculated the P-value . b , Expression analysis of HRH1 in PNI-annotated TCGA tumors. P-value calculated by linear regression after adjusting for cancer types. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. c , Release of histamine by mast cells investigated with toluidine blue at low pH in normal skin, PNI neg, and PNI pos cSCC. Deep purple-histamine-positive cells, light blue-background, scale bar=100μm, images are representative of n=5/group. d , Release of histamine by mast cells in normal biliary epithelium, PNI neg, and PNI pos CHOL. Deep purple-histamine-positive cells, light blue-background, scale bar=100μm, images are representative of n=5/group. e , Release of histamine by mast cells in normal oral mucosa, PNI neg, and PNI pos HNSC. Deep purple-positive cells, light blue-background, scale bar=100μm, images are representative of n=5/group. f , HRAS and CDK4 -transformed tumor spheroids were treated with either vehicle control, histamine, or a combination of histamine and H1-specific or H2-specific antihistamine. Tumor spheroid invasion was evaluated with live (green) and dead (red) staining and fluorescence microscopy. Scale bar=100μm. Results are representative of three independent experiments conducted in replicates. g , Immunofluorescence analysis of PNI organoids treated with either vehicle, histamine, or a combination of histamine and H1-specific or H2-specific antihistamine. The dotted line outlines the nerve sheath. Scale bar=100μm. Results are representative of three independent experiments conducted in replicates.

Journal: bioRxiv

Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

doi: 10.1101/2025.06.23.661147

Figure Lengend Snippet: a , Expression of various mast cell mediator receptors in keratinocyte populations of PNI neg and PNI pos cSCC assessed by scRNA-seq. Wilcoxon rank sum test calculated the P-value . b , Expression analysis of HRH1 in PNI-annotated TCGA tumors. P-value calculated by linear regression after adjusting for cancer types. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. c , Release of histamine by mast cells investigated with toluidine blue at low pH in normal skin, PNI neg, and PNI pos cSCC. Deep purple-histamine-positive cells, light blue-background, scale bar=100μm, images are representative of n=5/group. d , Release of histamine by mast cells in normal biliary epithelium, PNI neg, and PNI pos CHOL. Deep purple-histamine-positive cells, light blue-background, scale bar=100μm, images are representative of n=5/group. e , Release of histamine by mast cells in normal oral mucosa, PNI neg, and PNI pos HNSC. Deep purple-positive cells, light blue-background, scale bar=100μm, images are representative of n=5/group. f , HRAS and CDK4 -transformed tumor spheroids were treated with either vehicle control, histamine, or a combination of histamine and H1-specific or H2-specific antihistamine. Tumor spheroid invasion was evaluated with live (green) and dead (red) staining and fluorescence microscopy. Scale bar=100μm. Results are representative of three independent experiments conducted in replicates. g , Immunofluorescence analysis of PNI organoids treated with either vehicle, histamine, or a combination of histamine and H1-specific or H2-specific antihistamine. The dotted line outlines the nerve sheath. Scale bar=100μm. Results are representative of three independent experiments conducted in replicates.

Article Snippet: Cells were maintained at 37°C with 5% CO2 in medium 154 (Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific).

Techniques: Expressing, Transformation Assay, Control, Staining, Fluorescence, Microscopy, Immunofluorescence

a , Quantification of histamine-positive mast cells using toluidine blue in PNI neg and PNI pos cSCC, CHOL, and HNSC. A three-five field of view was used to quantify histamine-positive cells from images representing n=5/group. b-c , The Dose response of histamine treatments in keratinocytes was evaluated with HRH1 expression via qRT-PCR and immunoblotting. Conducted with replicates and repeated three independent times. Two-tailed Student’s t-tests calculated the P-value , error bar-standard deviation, Hist-histamine. d , Immunoblotting for P-JNK, JNK, and β-Actin upon histamine treatment in primary keratinocytes. Repeated three times. e , qRT-PCR analysis of HRH1 in different doses of H1-or H2-antihistamines following histamine treatment in keratinocytes compared to histamine group. Conducted with replicates and repeated three independent times. Two-tailed Student’s t-tests calculated the P-value , error bar-standard deviation, Hist-histamine, Niza-Nizatidine, CF-clemastine fumarate, Des-Desloratadine.

Journal: bioRxiv

Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

doi: 10.1101/2025.06.23.661147

Figure Lengend Snippet: a , Quantification of histamine-positive mast cells using toluidine blue in PNI neg and PNI pos cSCC, CHOL, and HNSC. A three-five field of view was used to quantify histamine-positive cells from images representing n=5/group. b-c , The Dose response of histamine treatments in keratinocytes was evaluated with HRH1 expression via qRT-PCR and immunoblotting. Conducted with replicates and repeated three independent times. Two-tailed Student’s t-tests calculated the P-value , error bar-standard deviation, Hist-histamine. d , Immunoblotting for P-JNK, JNK, and β-Actin upon histamine treatment in primary keratinocytes. Repeated three times. e , qRT-PCR analysis of HRH1 in different doses of H1-or H2-antihistamines following histamine treatment in keratinocytes compared to histamine group. Conducted with replicates and repeated three independent times. Two-tailed Student’s t-tests calculated the P-value , error bar-standard deviation, Hist-histamine, Niza-Nizatidine, CF-clemastine fumarate, Des-Desloratadine.

Article Snippet: Cells were maintained at 37°C with 5% CO2 in medium 154 (Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Two Tailed Test, Standard Deviation

Oncogenic HRAS and CDK4 -transformed primary keratinocytes were grown on human dermis at the air-liquid interface for 14 days and subjected to the drug treatments as shown. Immunofluorescence staining of keratin 5 (red), col VII (green), and nuclei (blue) was performed on the resulting cSCC organoids. Niza-Nizatidine, CF-clemastine fumarate, Des-Desloratadine. Scale bar=100μm, conducted with replicates and repeated three independent times.

Journal: bioRxiv

Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

doi: 10.1101/2025.06.23.661147

Figure Lengend Snippet: Oncogenic HRAS and CDK4 -transformed primary keratinocytes were grown on human dermis at the air-liquid interface for 14 days and subjected to the drug treatments as shown. Immunofluorescence staining of keratin 5 (red), col VII (green), and nuclei (blue) was performed on the resulting cSCC organoids. Niza-Nizatidine, CF-clemastine fumarate, Des-Desloratadine. Scale bar=100μm, conducted with replicates and repeated three independent times.

Article Snippet: Cells were maintained at 37°C with 5% CO2 in medium 154 (Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific).

Techniques: Transformation Assay, Immunofluorescence, Staining

a , Phosphoproteomic analysis of keratinocytes treated with either vehicle control or histamine for 10 minutes by mass spectrometry. The heatmap highlights the phosphorylation sites altered by histamine compared to the control. b , Pathway enrichment analysis by EnrichR of histamine phosphorylated sites in primary keratinocytes. c , Left, P38 gene signature enrichment in PNI-annotated TCGA tumors. Right, induction of various MMPs in PNI-positive TCGA tumors. P-value calculated by linear regression after adjusting for cancer types. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. d , Immunoblotting of P-P38, P38, and β-Actin in primary keratinocytes treated with vehicle control, hist-histamine, SB-SB203580 (P38 inhibitor) with histamine, or CF-clemastine fumarate (H1-antihistamine) with histamine. Results are representative of at least three independent experiments. e , Left, expression analysis of MMPs in primary keratinocytes treated with control, hist-histamine, SB-SB203580 (P38 inhibitor) with histamine, or CF-clemastine fumarate (H1-antihistamine) with histamine. Results are representative of at least three independent experiments conducted in replicates. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF and SB to the Hist group. Error bar-standard deviation. Right, ELISA for MMP2 and MMP9 in culture supernatants of primary keratinocytes in different treatment conditions. hist-histamine, SB-SB203580, CF-clemastine fumarate. Results are representative of multiple replicates in an experiment. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF and SB to the Hist group. Error bar-standard deviation. f , Live (green) and dead (red) staining in spheroids depicted tumor spheroid invasion upon the vehicle, histamine, or P38 inhibitor (SB203580) and histamine treatments. Scale bar=100μm, Results are representative of three independent experiments conducted in replicates. g , Immunofluorescence of Keratin 5 (red) displaying nerve invading oncogenic HRAS and CDK4 -transformed primary keratinocytes in PNI organoids treated with histamine, or P38 inhibitor (SB203580) and histamine. The dotted line outlines the nerve sheath. Scale bar=100μm, Results are representative of three independent experiments conducted in replicates.

Journal: bioRxiv

Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

doi: 10.1101/2025.06.23.661147

Figure Lengend Snippet: a , Phosphoproteomic analysis of keratinocytes treated with either vehicle control or histamine for 10 minutes by mass spectrometry. The heatmap highlights the phosphorylation sites altered by histamine compared to the control. b , Pathway enrichment analysis by EnrichR of histamine phosphorylated sites in primary keratinocytes. c , Left, P38 gene signature enrichment in PNI-annotated TCGA tumors. Right, induction of various MMPs in PNI-positive TCGA tumors. P-value calculated by linear regression after adjusting for cancer types. Error lines represent 1.5X interquartile range, and the box represents the interquartile range. d , Immunoblotting of P-P38, P38, and β-Actin in primary keratinocytes treated with vehicle control, hist-histamine, SB-SB203580 (P38 inhibitor) with histamine, or CF-clemastine fumarate (H1-antihistamine) with histamine. Results are representative of at least three independent experiments. e , Left, expression analysis of MMPs in primary keratinocytes treated with control, hist-histamine, SB-SB203580 (P38 inhibitor) with histamine, or CF-clemastine fumarate (H1-antihistamine) with histamine. Results are representative of at least three independent experiments conducted in replicates. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF and SB to the Hist group. Error bar-standard deviation. Right, ELISA for MMP2 and MMP9 in culture supernatants of primary keratinocytes in different treatment conditions. hist-histamine, SB-SB203580, CF-clemastine fumarate. Results are representative of multiple replicates in an experiment. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF and SB to the Hist group. Error bar-standard deviation. f , Live (green) and dead (red) staining in spheroids depicted tumor spheroid invasion upon the vehicle, histamine, or P38 inhibitor (SB203580) and histamine treatments. Scale bar=100μm, Results are representative of three independent experiments conducted in replicates. g , Immunofluorescence of Keratin 5 (red) displaying nerve invading oncogenic HRAS and CDK4 -transformed primary keratinocytes in PNI organoids treated with histamine, or P38 inhibitor (SB203580) and histamine. The dotted line outlines the nerve sheath. Scale bar=100μm, Results are representative of three independent experiments conducted in replicates.

Article Snippet: Cells were maintained at 37°C with 5% CO2 in medium 154 (Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific).

Techniques: Control, Mass Spectrometry, Phospho-proteomics, Western Blot, Expressing, Two Tailed Test, Standard Deviation, Enzyme-linked Immunosorbent Assay, Staining, Immunofluorescence, Transformation Assay

Review

human keratinocyte growth supplement (Thermo Fisher)

Structured Review

Images

Related Articles

Cell Culture:

Isolation:

Suspension:

Recombinant:

other:

Similar Products

Image Search Results